教育工作经历

1998-2002         武汉大学化学与分子科学学院         学士

2002-2008         中国科学院生物物理研究所          博士

2009-2015         美国国立卫生研究院国立癌症研究所      博士后

2015-至今         清华大学生命科学学院            研究员、博导

研究方向：  

课题组主要聚焦于RNA的整合结构生物学研究。近年来，我们发展了长链RNA的位点特异性标记技术（Nat.Commun. 2021，PNAS 2020，Chem. Sci. 2020），发展了长链RNA及其蛋白质复合物高级结构研究的整合方案；另一方面，我们整合运用小角X射线/中子散射、电子顺磁共振/核磁共振、X射线晶体学、单分子荧光共振能量转移/单分子生物纳米孔技术以及计算模拟，研究了黄病毒来源的功能RNA的高级结构、动态特性、相互作用与功能的关系（Biophys.J. 2022，Nat.Commun. 2021，Nat. Commun. 2020, EMBO Rep 2019）。此外，课题组在致病细菌功能性淀粉样蛋白的结构与形成机制方面也进行了有益探索(EMBO J. 2021，JBC 2018)。课题组将继续开发RNA结构研究的新方法，并应用相关方法开展与重大疾病和关键生命过程密切相关的非编码RNA的结构生物学研究，开展基于结构的mRNA分子的理性设计与优化研究。

发表论文

1. Burkhard Endeward^#, Yanping Hu^#, Guangcan Bai, Guoquan Liu, Thomas F. Prisner^*, Xianyang Fang^*. Long-range distance determination in fully deuterated RNA with pulsed EPR spectroscopy. Biophysical Journal2022，121(1):37-43.
2. Xiaolin Niu^#, Ruirui Sun^#, Zhifeng Chen, Yirong Yao, Xiaobing Zuo, Chunlai Chen^*, Xianyang Fang^*. Pseudoknot length modulates the folding, conformational dynamics and robustness of Xrn1 resistance of flaviviral xrRNAs. Nature Communications2021, 12(1): 6417.
3. Junfeng Ma, Xiang Cheng, Zhonghe Xu, Yikan Zhang, Jaione Valle, Shilong Fan, Xiaobing Zuo, Iñigo Lasa, Xianyang Fang^*. Structural mechanism for modulation of functional amyloid and biofilm formation by Staphylococcal Bap protein switch. The EMBO Journal2021, 40(14): e107500. (ResearchHighlight on Nature Chemical Biology 2021,17: 839)
4. Youqi Tao^#, Jingfei Xie^#, Qinglu Zhong^#, Yongyao Wang^#, Shengnan Zhang, Feng Luo, Fengcai Wen, Jingjing Xie, Jiawei Zhao, Xiaoou Sun, Houfang Long, Junfeng Ma, Qian Zhang, Jiangang Long, Xianyang Fang, Ying Lu, Dan Li, Ming Li, Jidong Zhu, Bo Sun^*, Guohui Li^*, Jiajie Diao^*, Cong Liu^*. A novel partially-open state of SHP2 points to a "multiple gear" regulation mechanism. Journal of Biological Chemistry2021, 296: 100538.
5. Jaione Valle, Xianyang Fang, Iñigo Lasa. Revisiting Bap multidomain protein: more than sticking bacteria together. Frontiers in Microbiology 2020, 11:613581.
6. Xiaolin Niu^#, Qiuhan Liu^#, Zhonghe Xu^#, Zhifeng Chen, Linghui Xu, Lilei Xu, Jinghong Li^*, Xianyang Fang^*. Molecular mechanisms underlying the mechanical anisotropy of flaviviral exoribonuclease-resistant RNAs (xrRNAs). Nature Communications2020, 11(1): 5496 (News and Views on Nature Chemical Biology 2021,17: 933-934)
7. Yan Wang, Venkatesan Kathiresan, Yaoyi Chen, Yanping Hu, Wei Jiang, Guangcan Bai, Guoquan Liu, Peter Z. Qin^*, Xianyang Fang.^*Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions.Chemical Science 2020, 11: 9655-9664.
8. Yan Wang, Yaoyi Chen, Yanping Hu, Xianyang Fang. Site-specific covalent labeling of large RNAs with nanoparticles empowered by expanded genetic alphabet transcription. Proceedings of the National Academy of Sciences USA2020,117(37): 22823-22832. (APS Science Highlights on APS Science 2020, Vol.2)
9. Yinlong Song, Yikan Zhang, Ying Pan, Jianfeng He, Yan Wang, Wei Chen, Jing Guo, Haiteng Deng, Yi Xue^*, Xianyang Fang^*, Xin Liang^*. The dimeric organization that enhances the microtubule end-binding affinity of EB1 is susceptible to phosphorylation. Journal of Cell Science2020，133(9): jcs241216.
10. Qiu-Yan Zhang^#, Xiao-Feng Li^#, Xiaolin Niu, Na Li, Hong-Jiang Wang, Cheng-Lin Deng, Han-Qing Ye, Xing-Yao Huang, Qi Chen, Yan-Peng Xu, Hao-Long Dong, Xiao-Dan Li, Hui Zhao, Pei-Yong Shi, Zhi-Ming Yuan, Peng Gong, Xianyang Fang, Cheng-Feng Qin^*, Bo Zhang^*. Short Direct Repeats in the 3′ Untranslated Region Are Involved in Subgenomic Flaviviral RNA Production. Journal of Virology2020，94(6): e01175-19.
11. Mengran Chen, Han Pan, Lingfei Sun, Peng Shi, Yikan Zhang, Le Li, Yuxing Huang, Jianhui Chen, Peng Jiang, Xianyang Fang, Congying Wu, Zhucheng Chen. Structure and regulation of human epithelial cell transforming 2 protein. Proceedings of the National Academy of Sciences USA 2020，117：1027-1035.
12. Yupeng Zhang^#, Yikan Zhang^#, Zhong-Yu Liu^#，Meng-Li Cheng^#, Junfeng Ma, Yan Wang, Cheng-Feng Qin^*, Xianyang Fang^*. Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Reports2019, 20(11):e47016.
13. Lilia Kaustov, Alexander Lemak, Hong Wu, Marco Faini, Lixin Fan, Xianyang Fang, Hong Zeng, Shili Duan, Abdellah Allali-Hassani, Fengling Li, Yong Wei, Masoud Vedadi, Ruedi Aebersold, Yunxing Wang, Scott Houliston, Cheryl H Arrowsmith. The MLL1 trimeric catalytic complex is a dynamic conformational ensemble stabilized by multiple weak interactions. Nucleic Acids Research2019, 47(17): 9433-9447.
14. Jingxia Lu, Jiao Li, Yuan Wu, Xianyang Fang, Jiapeng Zhu^*, Huan Wang^*. Characterization of the FMN-Dependent Cysteine Decarboxylase from Thioviridamide Biosynthesis. Organic Letters2019, 21(12): 4676-4679.
15. Yanxiang Zhao, Yikan Zhang, Jinguang Huang, Shanshan Wang, Long Yi, Xin Zhang, Min Xu,  Xianyang Fang^*, Junfeng Liu^*. The effect of phosphate ion on the ssDNA binding mode of MoSub1, a Sub1/PC4 homolog from rice blast fungus. Proteins: Structure, Function, and Genetics2019, 87(4): 257-264.
16. Yan Wang^#, Jingbo Jiang^#, Yachao Gao^#, Yang Sun, Jianfeng Dai, Yang Wu, Di Qu, Gang Ma, Xianyang Fang^*. Staphylococcus epidermidis small basic protein (Sbp) forms amyloid fibrils, consistent with its function as a scaffolding protein in biofilms. Journal of Biological Chemistry 2018, 293(37): 14296-14311.
17. Senyan Zhang, Panpan Zhou, Pengfei Wang, Yangyang Li, Liwei Jiang, Wenxu Jia, Han Wang, Angela Fan, Dongli Wang, Xuanling Shi, Xianyang Fang, Michal Hammel,Shuying Wang, Xinquan Wang, Linqi Zhang. Structural Definition of a Unique Neutralization Epitope on the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein. Cell Reports 2018, 24(2): 441-452.
18. Yanan Dong, Yajuan Mu, Yongchao Xie, Yupeng Zhang, Youyou Han, Yu Zhou, Wenhe Wang, Zihe Liu, Mei Wu, Hao Wang, Man Pan, Ning Xu, Cong-Qiao Xu, Maojun Yang, Shilong Fan, Haiteng Deng, Tianwei Tan, Xiaoyun Liu, Lei Liu, Jun Li, Jiawei Wang, Xianyang Fang, Yue Feng. Structural basis of ubiquitin modification by the Legionella effector SdeA. Nature2018, 557(7707): 674-678.
19. Yuba R. Bhandari, Lixin Fan, Xianyang Fang, George F. Zaki, Eric A. Stahlberg, Wei Jiang, Charles D. Schwieters, Jason R. Stagno, Yun-Xing Wang. Topological Structure Determination of RNA Using Small-Angle X-Ray Scattering. Journal of Molecular Biology2017, 429 (23): 3635-3649.
20. Xianyang Fang^*, Malgorzata Michnicka, Yikan Zhang, Yun-Xing Wang, Edward P. Nikonowicz^*. Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch. Biochemistry2017, 56(28): 3549-3558.
21. He Song, Xianyang Fang, Lan Jin, Gary X.Shaw, Yun-Xing Wang, Xinhua Ji^*. The Functional Cycle of Rnt1p: Five Consecutive Steps of Double-Stranded RNA Processing by a Eukaryotic RNase III. Structure2017, 25: 353-363.
22. Xian Xia^#, Xiaoyu Liu^#, Tong Li, Xianyang Fang, Zhucheng Chen^*. Structure of Chromatin Remodeller Swi2/Snf2 in the Resting State. Nature Structural and Molecular Biology2016, 23: 722-729.  
23. Smita Kakar^#, Xianyang Fang^#, Lucyna Lubkowska^#, Yan Ning Zhou, Gary X. Shaw, Yun-Xing Wang, Ding Jun Jin^*, Mikhail Kashlev^*, Xinhua Ji^*. Allosteric Activation of Bacterial Swi2/Snf2 (Switch/Sucrose Non-fermentable) Protein RapA by RNA Polymerase: BIOCHEMICAL AND STRUCTURAL STUDIES.Journal of Biological Chemistry2015, 290 (39): 23656-69. (^#equal contribution）
24. Robert A. Dick^*, Siddhartha A. K. Datta^*, Hirsh Nanda, Xianyang Fang, Yi Wen, Marilia Barros, Yun-Xing Wang, Alan Rein, Volker M. Vogt. Hydrodynamic and Membrane Binding Properties of Purified Rous Sarcoma Virus Gag Protein. Journal of Virololgy 2015, 89: 10371-10382.
25. Xianyang Fang, Jason R. Stagno, Yuba Bh.andari, Xiaobing Zuo, Yun-Xing Wang. Small-angle X-ray scattering: a bridge between RNA secondary structures and three-dimensional topological structures. Current Opinion in Structural Biology 2015,30: 147-160.
26. Sujay Subbayya Ithychanda, Xianyang Fang, Maradumane L. Mohan, Liang Zhu, Kalyan C. Tirupula, Sathyamangla V. Naga Prasad, Yun-Xing Wang, Sadashiva Karnik, Jun Qin. A mechanism of global shape-dependent recognition and phosphorylation of filamin by protein kinase A. Journal of Biological Chemistry2015, 290(13): 8527-38.
27. Yi Peng, Joseph E. Curtis, Xianyang Fang, Sarah A. Woodson. Structural Model of the folded rpoS mRNA leader in complex with Hfq. Proceedings of the National Academy of Sciences USA 2014, 111(48): 17134-9.
28. Alexander Lemak, Bin Wu, Adelinda Yee, Scott Houliston, Hsiau-Wei Lee,  Aleksandras Gutmanas, Xianyang Fang, Maite Garcia, Anthony Semesi,  Yun-Xing Wang, James H. Prestegard, Cheryl H. Arrowsmith. Structural characterization of a flexible two-domain proteinin solution based using Small Angle X-ray Scattering and NMR Data. Structure 2014, 22(12): 1862-74
29. Feng-Ming James Chang, Coyne, H. Jerome, Ramirez, Ciro Alberto Cubillas, Pablo Vinuesa Fleischmann, Xianyang Fang, Zhen Ma, Dejian Ma, John D. Helmann, Alejandro García-de los Santos, Yun-Xing Wang, Charles E. Dann III, David P. Giedroc. Cu(I)-mediated Allosteric Switching in a Copper-sensing Operon Repressor (CsoR). Journal of Biological Chemistry2014, 289(27): 19204-17.
30. Xianyang Fang^# , Jinbu Wang^#, Ina P. O’Carroll^#, Michelle Mitchell, Xiaobing Zuo, Yi Wang, Ping Yu, Yu Liu, Jason W. Rausch, Marzena A. Dyba, Jørgen Kjems, Charles D. Schwieters, Soenke Seifert, Randall E. Winans, Norman R. Watts, Stephen J. Stahl, Paul T. Wingfield, R. Andrew Byrd, Stuart F. J. Le Grice, Alan Rein^*, Yun-Xing Wang^*. An unusual topological structure of the HIV-1 rev response element, Cell 2013, 155(3): 594-605.( ^#equal contribution)
31. Moshe Giladi, Itay Friedberg, Xianyang Fang, Reuben Hiller, Yun-Xing Wang, Daniel Khananshvili. G503 is obligatory for dynamic coupling of regulatory domains in NCX proteins Biochemistry 2012, 51(37): 7313-20.
32. Moshe Giladi, Yeheskel Sasson, Xianyang Fang, Reuben Hiller, Tal Buki, Yun-Xing Wang, Joel Alan Hirsch, Daniel Khananshvili. A common Ca²⁺-driven interdomain module governs eukaryotic NCX regulation, PLoS One 2012, 7(6): e39985.
33. George Avvakumov, John Walker, Sheng Xue, Abdellah Allali-Hassani, Abdalin Asinas, Usha Nair, Xianyang Fang, Xiaobing Zuo, Yun-Xing Wang, Keith Wilkinson, Sirano Dhe-Paganon. Two ZnF-UBP domains in isopeptidase T (USP5), Biochemistry 2012, 51(6): 1188-98.
34. Xianyang Fang, Yingang Feng, Jinfeng Wang. Favorable contribution of the C-terminal residue K97 to the stability of a hyperthermophilic archaeal [P62A]Ssh10b, Archives of Biochemistry and Biophysics 2009, 481(1): 52-8.
35. Xianyang Fang^#, Qiu Cui^#, Yufeng Tong, Yingang Feng, Lu Shan, Li Huang, Jinfeng Wang. A stabilizing a/β-hydrophobic core greatly contributes to hyperthermostability of archaeal [P62A]Ssh10b. Biochemistry 2008, 47(43): 11212-21.
36. Quan Luo^#, Xianyang Fang, Zhiwei Deng, Jinfeng Wang. NMR study of influences of D51N mutation on backbone conformation of hyperthermophilic archaeal [P62A]Ssh10b. Chemical  Journal of Chinese Universities-Chinese 2008, 29: 546-550

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