秦磊

 

 

秦磊

清华大学 化工系

助理研究员

 

 

  教育经历 

2005.9 ~ 2009.6         天津大学化工学院                        学士                 

2009.9 ~ 2012.6         天津大学化工学院                        硕士           

2012.7 ~ 2013.8        天津大学化工学院                        科研助理

2013.9 ~ 2017.6         天津大学化工学院                        博士               

2017.10 ~ 2020.11     北京理工大学化学与化工学院    博士后            

2020.12 ~ 2023.01     清华大学化工系                            博士后          

       2023.3 ~ 至今            清华大学化工系                            助理研究员

 

研究方向

  主要从事工业发酵菌株抗逆性和鲁棒性的系统改造，采用系统生物学和合成生物学的方法，在提升工业菌株的鲁棒性、抗热、抗酸碱和抗氧化性，提高菌株对发酵环境的智能响应能力，提高外源基因线路与底盘细胞适配性，以及酵母基因组代谢网络模型与抗逆网络模型构建等方面做出了开拓性工作，以第一作者或通讯作者在Metab Eng、ACS Energ Lett、Bioresour Technol等顶级期刊上发表论文40篇，申请发明专利15项（授权5项），改造的菌株在企业实现技术转化。

 

代表性论文

    1. Qin L, Ma D, Lin G, Sun W, Li C. Low temperature promotes the production and efflux of terpenoids in yeast. Bioresource Technology 2024, 24:130376.
    2. Liu X#, Qin L#, Yu J, Sun W, Xu J, Li C*. Real-time monitoring of subcellular states with genetically encoded redox biosensor system (RBS) in yeast cell factories. Biosensors and Bioelectronics 2023, 222:114988.
    3. Qin L, Liu X, Xu K, Li C*. Mining and design of biosensors for engineering microbial cell factory. Current Opinions in Biotechnology 2022, 75:102694.
    4. Lv X, Xue H, Qin L*, Li C*. Transporter engineering in microbial cell factory boosts biomanufacturing capacity. BioDesign Research 2022, 9871087.
    5. Gao X, Xu K, Ahmad N, Qin L*, Li C. Recent advances in engineering of microbial cell factories for intelligent pH regulation and tolerance. Biotechnology Journal 2021, 24:e2100151.
    6. Qin L, Dong S, Yu J, Ning X, Xu K, Zhang SJ, Xu L, Li BZ, Li J, Yuan YJ, Li C*. Stress-driven dynamic regulation of multiple tolerance genes improves robustness and productive capacity of Saccharomyces cerevisiae in industrial lignocellulose fermentation. Metabolic Engineering 2020, 61:160-170.
    7. Xu K#, Qin L#, Bai W, Wang X, Li F, Ren S, Gao X, Chen B, Tong Y, Li J, Li BZ, Yuan YJ, Li C*. Multilevel defense system (MDS) relieves multiple stresses for economically boosting ethanol production of industrial Saccharomyces cerevisiae. ACS Energy Letters 2020, 5:572-582.
    8. Huangfu J1, Kim HS1, Xu K, Ning X, Qin L*, Li J*, Li C. Omics analysis reveals the mechanism of enhanced recombinant protein production under simulated microgravity. Frontiers in Bioengineering and Biotechnology 2020, 8:30.
    9. Qin L#, Zhao X#, Li WC, Zhu JQ, Liu L, Li BZ*, Yuan YJ. Process analysis and optimization of simultaneous saccharification and co‑fermentation of ethylenediamine‑pretreated corn stover for ethanol production. Biotechnology for Biofuels 2018, 11:118.
    10. Qin L#, Li X#, Liu L, Zhu JQ, Guan QM, Zhang MT, Li WC, Li BZ*, Yuan YJ. Dual effect of soluble materials in pretreated lignocellulose on simultaneous saccharification and co-fermentation process for the bioethanol production. Bioresource Technology 2017, 224:342-8.
    11. Qin L#, Li X#, Zhu JQ, Li WC, Xu H, Guan QM, Zhang MT, Li BZ*, Yuan YJ. Optimization of ethylenediamine pretreatment and enzymatic hydrolysis to produce fermentable sugars from corn stover. Industrial Crops and Products 2017, 102:51-7.
    12. Qin L, Li WC, Liu L, Zhu JQ, Li X, Li BZ*, Yuan YJ. Inhibition of lignin-derived phenolic compounds to cellulase. Biotechnology for Biofuels2016, 9:70.
    13. Qin L, Liu L, Li WC, Zhu JQ, Li BZ*, Yuan YJ. Evaluation of soluble fraction and enzymatic residual fraction of dilute dry acid, ethylenediamine, and steam explosion pretreated corn stover on the enzymatic hydrolysis of cellulose. Bioresource Technology 2016, 209:172-9.
    14. Qin L, Li WC, Zhu JQ, Liang JN, Li BZ*, Yuan YJ. Ethylenediamine pretreatment changes cellulose allomorph and lignin structure of lignocellulose at ambient pressure. Biotechnology for Biofuels 2015, 8:174.
    15. Liu ZH#, Qin L#, Li BZ*, Yuan YJ. Physical and chemical characterization of corn stover from leading pretreatment methods and effects on enzymatic hydrolysis. ACS Sustainable Chemistry and Engineering2015, 3:140-6.
    16. Qin L, Liu ZH, Jin M, Li BZ, Yuan YJ*. High temperature aqueous ammonia pretreatment and post-washing enhance the high solids enzymatic hydrolysis of corn stover. Bioresource Technology2013, 146:504-11.
    17. Qin L, Liu ZH, Li BZ, Dale BE, Yuan YJ*. Mass balance and transformation of corn stover by pretreatment with different dilute organic acids. Bioresource Technology 2012, 112:319-26.
    18. 秦磊, 俞杰, 宁小钰, 孙文涛, 李春. 合成生物系统构建与绿色生物“智”造. 化工学报, 2020, 71(9): 3979-3994.
    19. Wentao Sun, Shengtong Wan, Chuyan Liu, Ruwen Wang, Haocheng Zhang, Lei Qin, Bo Lv*, Chun Li*. Establishing cell suitability for high-level production of licorice triterpenoids in yeast. 2024. Acta Pharmaceutica Sinica B.
    20. Ting An, Guangyuan Lin, Yang Liu, Lei Qin, Yuquan Xu, Xudong Feng*, Chun Li*. De novo biosynthesis of icariin in engineered yeast. Metabolic Engineering. 2023, 80:207-215.
    21. 许可,张运峰,秦磊,范永山,李春. 耐热基因线路随机组装构建高版本耐热酿酒酵母. 生命科学, 2023, 53(3):399-407
    22. 董曙馨秦磊李春李珺* 利用转录因子工程重塑代谢网络实现细胞工厂高效生产中国生物工程杂志 2021, 41(4):55-63.
    23. 王靖楠，庞建，秦磊，郭超，吕波，李春*，王超*．丁烯基多杀菌素高产菌株的选育和改造策略．化工学报 2022, 73(2):566-576.
    24. 张震，曾雪城，秦磊，李春*．微生物细胞工厂的智能设计进展．化工学报, 2021, 72(12):6093-6108.
    25. 刘夏，秦磊，李春*. 细胞工厂底盘抗逆属性的分子调控进展[J]. 生命科学, 2021, 33(12):1452-1461.
    26. Sun Wentao, Qin Lei, Xue Haijie, Yu Yang, Ma Yihua, Wang Ying & Li Chun*. Novel trends for producing plant triterpenoids in yeast, Critical Reviews in Biotechnology, 2019, 39(5):618-632.
    27. Ni Jiangping#, Zhang Gen-Lin#, Qin Lei, Li Jun*, Li Chun*, Simultaneously down-regulation of multiplex branch pathways using CRISPRi and fermentation optimization for enhancing beta-amyrin production in Saccharomyces cerevisiae. Synth. Syst. Biotechnol. 2019, 4, 79-85.
    28. Ding, M. Z., Chen, B. Q., Ji, X. J., Zhou, J. W., Wang, H. Y., Tian, X. W., Feng, X. D., Yue, H., Zhou, Y. J., Wang, H. L., Wu, J. P., Yang, P. P., Jiang, Y., Mao, X. M., Xiao, G., Zhong, C., Xiao, W. H., Li, B. Z., Qin, L., Cheng, J. S., Yao, M. D., Wang, Y., Liu, H., Zhang, L., Yu, L. L., Chen, T., Dong, X. Y., Jia, X. Q., Zhang, S. P., Liu, Y. F., Chen, Y., Chen, K. Q., Wu, J. L., Zhu, C. J., Zhuang, W., Xu, S., Jiao, P. F., Zhang, L., Song, H., Yang, S., Xiong, Y., Li, Y. Q., Zhang, Y. M., Zhuang, Y. P., Su, H. J., Fu, W. P., Huang, Y. M., Li Chun, Zhao, Z. B. K., Sun, Y., Chen, G. Q., Zhao, X. M., Huang, H., Zheng, Y. G., Yang, L. R., Su, Z. G., Ma, G. H., Ying, H. J., Chen, J., Tan, T. W., and Yuan, Y. J. Biochemical engineering in China. Rev. Chem. Eng. 2019, 35, 929-993.
    29. 王小艳, 秦磊, 刘辉, 李凡, 李春, 佟毅*. 淀粉质燃料乙醇发酵胁迫及菌株耐受性改造. 精细化工 2019, 36, 568-574.
    30. Li, WC; Zhu, JQ; Zhao, X; Qin, L; Xu, T; Zhou, X; Li, X; Li, BZ; Yuan, YJ, Improving co-fermentation of glucose and xylose by adaptive evolution of engineering xylose-fermenting Saccharomyces cerevisiae and different fermentation strategies. Renewable Energy, 2019, 139:1176-1183.
    31. Li, WC; Li, X; Zhu, JQ; Qin, L; Li, BZ; Yuan, YJ. Improving xylose utilization and ethanol production from dry dilute acid pretreated corn stover by two-step and fed-batch fermentation. ENERGY. 2018, 157:877-885.
    32. Zhu, JQ, Li, WC, Qin, L, Zhao, X, Chen, S, Liu, H, Liu, ZH, Zhou, X, Li, X, Li, BZ, Yuan, YJ. Stepwise pretreatment of aqueous ammonia and ethylenediamine improve enzymatic hydrolysis of corn stover. Industrial Crops and Products. 2018, 124:201-208.
    33. Li WC1, Li X1, Qin L, Zhu JQ, Han X, Li BZ*, Yuan YJ. Reducing sugar loss in enzymatic hydrolysis of ethylenediamine pretreated corn stover, Bioresource Technology 2017, 224:405-10. (IF=4.917)
    34. Li, CC; Lin, FM; Zhou, L; Qin, L; Li, BZ; Zhou, ZH; Jin, MJ; Chen, Z. Cellulase hyper-production by Trichoderma reesei mutant SEU-7 on lactose. Biotechnology for Biofuels. 2017, 10:228
    35. Zhu JQ1, Li X1, Qin L, Li WC, Li HZ, Li BZ*, Yuan YJ. In situ detoxification of dry dilute acid pretreated corn stover by co-culture of xylose-utilizing and inhibitor-tolerant Saccharomyces cerevisiae increases ethanol production. Bioresource Technology 2016, 218:380-7.
    36. Zhu JQ, Qin L, Li WC, Zhang J, Bao J, Huang YD, Li BZ*, Yuan YJ. Simultaneous saccharification and co-fermentation of dry dilute acid pretreated corn stover at high dry matter loading: Overcoming the inhibitors by non-tolerate yeast. Bioresource Technology 2015, 198:39-46.
    37. Zhu JQ, Qin L, Li BZ*, Yuan YJ. Simultaneous saccharification and co-fermentation of aqueous ammonia pretreated corn stover with an engineered Saccharomyces cerevisiae SyBE005. Bioresource Technology 2014, 169:9-18.
    38. Liu ZH, Qin L, Zhu JQ, Li BZ*, Yuan YJ. Simultaneous saccharification and fermentation of steam-exploded corn stover at high glucan loading and high temperature. Biotechnology for Biofuels 2014, 7:167.
    39. Feng L, Qin L, Liu ZH, Dong CY, Li BZ*, Yuan YJ. Combined severity during pretreatment chemical and temperature on the saccharification of wheat straw using acids and alkalis of differing strength. BioResources 2014, 9:24-38.
    40. Liu ZH, Qin L, Jin MJ, Pang F, Li BZ, Kang Y, Dale BE, Yuan YJ*. Evaluation of storage methods for the conversion of corn stover biomass to sugars based on steam explosion pretreatment. Bioresource Technology 2013, 132:5-15.
    41. Liu ZH, Qin L, Pang F, Jin MJ, Li BZ, Kang Y, Dale BE, Yuan YJ*. Effects of biomass particle size on steam explosion pretreatment performance for improving the enzyme digestibility of corn stover. Industrial Crops and Products 2013, 44:176-84.
    42. 元英进*, 秦磊, 李炳志, 艾瑞婷. 乙醇预处理对水稻秸秆物质迁移和酶解的影响. 天津大学学报 2012, 45(9):757-62.

 

专利发明

  1. 元英进；秦磊；李炳志；刘志华，一种提高木质纤维素酶解糖化效率的方法
  2. 元英进；秦磊；李文超；李炳志；朱家庆，一种微生物发酵乙醇的方法
  3. 元英进；秦磊；李文超；李炳志；朱家庆，一种木质纤维素原料预处理的方法及应用
  4. 元英进；秦磊；李文超；李炳志；朱家庆，一种木质纤维素原料预处理的方法及应用
  5. 佟毅; 李春; 王小艳; 许可; 何太波; 王康; 秦磊; 白文鑫; 李义; 李凡; 袁敬伟; 刘辉; 陈博，一株耐高温的酿酒酵母菌株及其应用
  6. 秦磊；李春，一种提高酿酒酵母鲁棒性的菌株构建方法
  7. 李春；秦磊；刘夏，提高菌株抗逆性的方法及基因在提高菌株抗逆性中的应用
  8. 李春；刘夏；秦磊，一种生物传感器、核酸分子、表达载体及应用
  9. 高慧鹏，秦磊，张亚鹏，张全，李春. 一种提高酵母合成石竹烯能力的方法
  10. 李春，张亚鹏，高慧鹏，张全，秦磊. 一种生产石竹烯的酿酒酵母工程菌株及其应用
  11. 关浩，秦磊，张亚鹏，张全，李春. 一种生产蒎烯的酿酒酵母工程菌株及其应用
  12. 李春，马治东，王蒙，秦磊，张全. 一种生产红没药烯的酿酒酵母工程菌株及其应用
  13. 李春，张亚鹏，吕波，秦磊. 一种生产石竹烯的基因工程菌株及其构建方法
  14. 李春，孙文涛，秦磊. 一种工程菌及其在合成稀有甘草三萜中的应用
  15. 李春，孙文涛，秦磊. 11-氧-β-香树脂醇氧化酶 CYP72A63 突变体、基因及应用

 

论著

  1. Qin L, Li WC, Zhu JQ, Li BZ*, Yuan YJ. Chapter 1. Hydrolysis of Lignocellulosic Biomass to Sugars. In: Fang Z., Smith, Jr. R., Qi X. (eds) Production of Platform Chemicals from Sustainable Resources. Biofuels and Biorefineries. 2017. Springer Nature Singapore.
  2. 编委会成员，《化学工程发展战略：高端化、绿色化、智能化》，张锁江等主编，北京：化学工业出版社，2023年1月
  3. 编委会成员，《中国合成生物学2035发展战略》，科学出版社，2023年5月

 

联系方式

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